Restorative neurology and neuroscience · 2004
Do peripheral nerve extracts protect retinal ganglion cells from axotomy-induced death?
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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История · было → стало
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Swiech-Sabuda Ewa, Gołka Beata, Kotulska Katarzyna, Larysz-Brysz Magdalena, Górka Dariusz, Gołka Dariusz, Marcol Wiesław, Lewin-Kowalik Joanna
Аннотация
PURPOSE: Study the effect of peripheral nerve extracts and BDNF on retinal ganglion cell (RGC) regeneration in adult rats.
METHODS: Postmicrosomal fractions were obtained from 7-day, predegenerated, as well as non-predegenerated peripheral adult rats nerves. Autologous connective tissue chambers filled with fibrin were implanted into a gap-injury site in the optic nerve. Chambers contained either fibrin, the fractions, or BDNF. RGCs retrogradely labeled with DiI were counted, the distance covered by regrowing fibers traced with rhodamine B was measured, and number of myelinated fibers in the middle part of the chambers was estimated.
RESULTS: BDNF and predegenerated nerve extracts enhanced RGC fiber outgrowth. The regeneration was significantly weaker in the groups with non-predegenerated nerve extracts, as well as fibrin, and was absent in the control group.
CONCLUSION: Predegenerated nerve extracts, as well as BDNF, applied to injured optic nerves by means of connective tissue chambers, are able to induce retinal ganglion cell regeneration.
METHODS: Postmicrosomal fractions were obtained from 7-day, predegenerated, as well as non-predegenerated peripheral adult rats nerves. Autologous connective tissue chambers filled with fibrin were implanted into a gap-injury site in the optic nerve. Chambers contained either fibrin, the fractions, or BDNF. RGCs retrogradely labeled with DiI were counted, the distance covered by regrowing fibers traced with rhodamine B was measured, and number of myelinated fibers in the middle part of the chambers was estimated.
RESULTS: BDNF and predegenerated nerve extracts enhanced RGC fiber outgrowth. The regeneration was significantly weaker in the groups with non-predegenerated nerve extracts, as well as fibrin, and was absent in the control group.
CONCLUSION: Predegenerated nerve extracts, as well as BDNF, applied to injured optic nerves by means of connective tissue chambers, are able to induce retinal ganglion cell regeneration.