In Vitro Anti-Inflammatory Terpenoid Glycosides from the Seeds of Dolichos lablab.

To further explore the anti-inflammatory components of the seeds of Dolichos lablab L., a comprehensive phytochemical investigation was conducted using diverse chromatographic and spectrometric technologies, as well as chemical reactions. As a result, ten previously unreported terpenoid glycosides, namely dolilabterpenosides A, B, C1-C3, D, E, and F1-F3 (1-10), along with four known analogues (11-14), initially identified from Dolichos genus, were obtained. In addition, the lipopolysaccharide (LPS)-induced RAW264.7 cell model was employed to detect the expression levels of nitric oxide (NO), inflammatory cytokines tumour necrosis factor (TNF)-α and interleukin (IL)-1β to assess the anti-inflammatory activities of the obtained compounds. The results of bioactive assay showed that compounds 1, 4-7, and 10-12 showed significant inhibitory activity on NO release in RAW264.7 cells in a dose-dependent manner, and all of them were demonstrated to inhibit the increase in TNF-α and IL-Iβ levels in the supernatant of RAW264.7 cells stimulated by LPS.