Effects of acute doses of ethanol administered at pre-implantation stages on fetal development in the mouse.

The objective of the present study was to investigate the effects of maternal administration of acute doses of alcohol at preimplantation stages of pregnancy on the fetus and fetal membranes. Single doses of 0.02 ml and 0.03 ml/g body wt. of 25% v/v of absolute alcohol in saline were administered to groups of MF1 mice on one of days 1-6 of gestation. Controls were either pair-fed and pair-watered or saline-treated. Fetuses and placentae were collected on day 15. Resorption and fetal death were increased in most of the alcohol treatment groups. The reduction in fetal body weight was significant in the 0.03 ml group. Compared to the saline controls, most experimental groups exhibited a rise in placental weight. The reduction in cord length was significant in comparison to untreated controls. Craniofacial and systemic abnormalities appeared to correlate with retardation of fetal growth. Hence, following treatment of 0.03 ml/g body wt. of alcohol solution on days 2 and 6, fetuses were collected on days 14, 16 and 18. Most fetuses were found to recover gradually from anomalies associated with growth retardation but the 'catch up' growth was not adequate enough to compensate for the reduction in body weight. These observations indicate that the pre-implantation stage of mouse embryo development is susceptible to the deleterious effects (fetal death and intrauterine growth retardation) of acute doses of alcohol.