Zhonghua yi xue za zhi · 2012
[Expression of proteasome β5 subunit in human atherosclerotic plaque].
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Wang Ming-xiao, Shi Ning, Wang Chun-ling, Zhang Ning, Ping Xiao-jia, Liu Yan-lin, Zhang Jian-mei
Аннотация
OBJECTIVE: To evaluate the expression of proteasome β5 subunit in human atherosclerotic plaque.
METHODS: The specimens of carotid endarterectomy were collected from 16 carotid stenosis patients. For the case group, they were divided into 2 groups: plaque (group A) and areas adjacent to plaque (group B). And carotid endarterium was obtained from 6 patients undergoing aortic replacement surgery. Relatively normal arterial intima (subclavian artery & innominate artery) was selected as the control group (group C). Content of proteasome β5 subunit was evaluated by hematoxylin & eosin (HE) staining, immunohistochemistry and Western blot.
RESULTS: (1) In patients with diabetes, hyperlipidemia and probable smoking, proteasome β5 subunit was enhanced in all groups; (2) Proteasome β5 subunit was demonstrated in both cytoplasm and nucleus of vascular smooth muscle cells, but mainly confined to cytoplasm; (3)The positive expression rate of β5 was 9/16 in group A, 11/16 in group B and 6/6 in group C. There was significant divergence in the expression of β5 in different sample sub-groups: group A (atherosclerotic plaque) < group B (areas adjacent to plaque) < group C (control group).
CONCLUSION: The expression of β5 decrease markedly in human atherosclerotic plaques.
METHODS: The specimens of carotid endarterectomy were collected from 16 carotid stenosis patients. For the case group, they were divided into 2 groups: plaque (group A) and areas adjacent to plaque (group B). And carotid endarterium was obtained from 6 patients undergoing aortic replacement surgery. Relatively normal arterial intima (subclavian artery & innominate artery) was selected as the control group (group C). Content of proteasome β5 subunit was evaluated by hematoxylin & eosin (HE) staining, immunohistochemistry and Western blot.
RESULTS: (1) In patients with diabetes, hyperlipidemia and probable smoking, proteasome β5 subunit was enhanced in all groups; (2) Proteasome β5 subunit was demonstrated in both cytoplasm and nucleus of vascular smooth muscle cells, but mainly confined to cytoplasm; (3)The positive expression rate of β5 was 9/16 in group A, 11/16 in group B and 6/6 in group C. There was significant divergence in the expression of β5 in different sample sub-groups: group A (atherosclerotic plaque) < group B (areas adjacent to plaque) < group C (control group).
CONCLUSION: The expression of β5 decrease markedly in human atherosclerotic plaques.