Fertility and sterility · 2007
The effect of interleukin-6 on ciliary beat frequency in the human fallopian tube.
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Papathanasiou A, Djahanbakhch O, Saridogan E, Lyons R A
Аннотация
OBJECTIVE: To investigate the effect of interleukin-6 (IL-6) on ciliary activity within the human fallopian tube in vitro.
DESIGN: An experimental laboratory-based study.
SETTING: University teaching hospital.
PATIENT(S): Fallopian tube specimens were obtained from eight women undergoing hysterectomy for fibroid uterus.
MAIN OUTCOME MEASURE(S): Ciliary beat frequency (CBF) in the human fallopian tube.
INTERVENTION(S): After baseline CBF measurements, increasing concentrations of IL-6 (10 pg/mL, 100 pg/mL, and 1000 pg/mL) were applied to fallopian tube mucosa explants and CBF measurements repeated. An anti-IL-6 monoclonal antibody was added and CBF measured once more. Negative and antibody-only control samples were used.
RESULT(S): The CBF remained unchanged with the addition of IL-6 at concentrations of 10 pg/mL and 100 pg/mL but IL-6 at 1000 pg/mL significantly decreased CBF, an effect abolished by the addition of the specific antibody.
CONCLUSION(S): These data suggest that IL-6 has an inhibitory effect on ciliary activity, suggesting a possible role for this cytokine in the subfertility process. This finding may be important in conditions where there are increased IL-6 levels in the peritoneal fluid, such as endometriosis and pelvic inflammatory disease.
DESIGN: An experimental laboratory-based study.
SETTING: University teaching hospital.
PATIENT(S): Fallopian tube specimens were obtained from eight women undergoing hysterectomy for fibroid uterus.
MAIN OUTCOME MEASURE(S): Ciliary beat frequency (CBF) in the human fallopian tube.
INTERVENTION(S): After baseline CBF measurements, increasing concentrations of IL-6 (10 pg/mL, 100 pg/mL, and 1000 pg/mL) were applied to fallopian tube mucosa explants and CBF measurements repeated. An anti-IL-6 monoclonal antibody was added and CBF measured once more. Negative and antibody-only control samples were used.
RESULT(S): The CBF remained unchanged with the addition of IL-6 at concentrations of 10 pg/mL and 100 pg/mL but IL-6 at 1000 pg/mL significantly decreased CBF, an effect abolished by the addition of the specific antibody.
CONCLUSION(S): These data suggest that IL-6 has an inhibitory effect on ciliary activity, suggesting a possible role for this cytokine in the subfertility process. This finding may be important in conditions where there are increased IL-6 levels in the peritoneal fluid, such as endometriosis and pelvic inflammatory disease.