Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan · 2006
[Detection of short tandem repeat (STR) polymorphisms by microchip electrophoresis for individual identification of cattle].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Yamaguchi Akihiro, Shimizu Kaori, Mishima Takashi, Hattori Hideki, Katsuda Shin-ichi, Sato Hidetaka, Ueda Nobuo, Sato Noriyuki
Аннотация
A simple and rapid detection of short tandem repeat (STR) markers was studied as a screening test for individual identification of cattle. DNAs were extracted from eight commercial beef samples by a proteinase K-boil method followed by purification with 2-propanol precipitation. Five STR markers, known to be highly polymorphic, were amplified by PCR and analyzed both by a conventional sequencing analysis (SEQ) and by a proposed microchip electrophoresis (MEP). Every marker revealed high polymorphism, such as 5-9 alleles in SEQ analysis, and 4-6 alleles in MEP analysis. This simple and rapid MEP analysis is expected to be an effective screening tool with use of confirmatory SEQ analysis.