Georgian medical news · 2025
ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF PLANT EXTRACTS: PROSPECTS FOR THE DEVELOPMENT OF COMBINED THERAPEUTIC AGENTS.
СтатьяНазвание и аннотация: Перевод в очереди
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Sharofova M, Khalimova F, Maidina H, Zhao J, Aisa H
Аннотация
INTRODUCTION: The aim of this study was to investigate the antimicrobial and anti-inflammatory activities of six samples (S1-S6) against pathogenic microorganisms (Staphylococcus aureus, Escherichia coli, and Candida albicans) and their potential to inhibit inflammatory processes. The primary objectives included identifying the most active compounds, determining their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), and evaluating their prospects for use in combined therapy of infectious and inflammatory diseases. The importance of this research arises from the growing issue of antibiotic resistance and the necessity of discovering novel therapeutic agents with dual (antimicrobial and anti-inflammatory) activities capable of simultaneously combating infections and reducing inflammation.
MATERIALS AND METHODS: The study was conducted following ethical standards established by the Helsinki Declaration of the World Medical Association and Good Laboratory Practice (GLP). Six samples (S1-S6) were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 100 mg/mL. Antimicrobial activity was assessed using reference strains of microorganisms: Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC11229), and Candida albicans (ATCC10231). Ampicillin (for bacteria) and Amphotericin B (for fungi) served as positive controls. Antimicrobial activity was evaluated through agar diffusion and broth dilution methods (MIC and MBC). Anti-inflammatory activity was determined by measuring the samples' capacity to suppress nitric oxide (NO) secretion in the Raw264.7 macrophage cell line. Statistical analysis was performed using ANOVA followed by Tukey's test, with significance set at p<0.05.
RESULTS: Sample S2 demonstrated the highest antimicrobial and anti-inflammatory activity, with inhibition zones up to 20 mm, MIC=7.8125 µg/mL, MBC=15.625 µg/mL, and significant suppression of nitric oxide (NO) production (75%, IC50=20 µM). Samples S1 and S3 showed moderate antimicrobial activity (MIC=31.25 µg/mL) and NO suppression levels ranging between 60-70%. Samples S4-S6 did not exhibit significant antimicrobial effects. The obtained results highlight the potential of sample S2 for further research and its possible application in treating infectious and inflammatory diseases.
CONCLUSION: Sample S2 exhibits significant antimicrobial and anti-inflammatory properties, making it a promising candidate for the development of novel therapeutic agents, particularly important in the context of growing antibiotic resistance and inflammatory diseases.
MATERIALS AND METHODS: The study was conducted following ethical standards established by the Helsinki Declaration of the World Medical Association and Good Laboratory Practice (GLP). Six samples (S1-S6) were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 100 mg/mL. Antimicrobial activity was assessed using reference strains of microorganisms: Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC11229), and Candida albicans (ATCC10231). Ampicillin (for bacteria) and Amphotericin B (for fungi) served as positive controls. Antimicrobial activity was evaluated through agar diffusion and broth dilution methods (MIC and MBC). Anti-inflammatory activity was determined by measuring the samples' capacity to suppress nitric oxide (NO) secretion in the Raw264.7 macrophage cell line. Statistical analysis was performed using ANOVA followed by Tukey's test, with significance set at p<0.05.
RESULTS: Sample S2 demonstrated the highest antimicrobial and anti-inflammatory activity, with inhibition zones up to 20 mm, MIC=7.8125 µg/mL, MBC=15.625 µg/mL, and significant suppression of nitric oxide (NO) production (75%, IC50=20 µM). Samples S1 and S3 showed moderate antimicrobial activity (MIC=31.25 µg/mL) and NO suppression levels ranging between 60-70%. Samples S4-S6 did not exhibit significant antimicrobial effects. The obtained results highlight the potential of sample S2 for further research and its possible application in treating infectious and inflammatory diseases.
CONCLUSION: Sample S2 exhibits significant antimicrobial and anti-inflammatory properties, making it a promising candidate for the development of novel therapeutic agents, particularly important in the context of growing antibiotic resistance and inflammatory diseases.