American journal of veterinary research · 2026
Storage affects allogenic posttransfusion survival of red blood cells in healthy goats.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Tseng Charissa Y, Castellanos Julyssa, Heller Meera C, Kretsch-York Cileah M, Skebba Hayley S, Panjwani Anushka, Burges Julie W, Chigerwe Munashe
Аннотация
OBJECTIVE: To evaluate the feasibility of biotin labeling of caprine RBCs and determine the posttransfusion survival of allogenic fresh and stored RBCs in healthy goats.
METHODS: Fifteen 13- to 15-month-old nonlactating goats were randomly assigned to 3 groups (5/group). Each goat served as a donor and recipient and was administered a whole-blood transfusion from a randomly assigned herd mate. The RBCs in whole blood were biotin labeled. Group 1 goats were transfused with fresh biotin-labeled whole blood within 6 hours of collection. Group 2 and 3 goats were transfused with biotin-labeled whole blood stored at 4 °C for 14 and 28 days, respectively. Blood samples were collected at 15 minutes and 1, 2, 3, 5, 7, 14, 21, and 28 days after transfusion. Biotin-labeled RBCs were detected using flow cytometry, and their half-lives were determined using nonlinear regression. The study was conducted from June 10 through September 2, 2025.
RESULTS: Biotin-labeled RBCs were distinctly detected by flow cytometry. The half-lives of biotin-labeled RBCs in goats in groups 1, 2, and 3 were 4.6, 4.3, and 2.5 days, respectively. The half-lives of biotin-labeled RBCs in groups 1 and 2 were longer than in group 3 (Akaike information criterion difference, > 2). There was no difference in biotin-labeled RBC half-lives between groups 1 and 2 (Akaike information criterion difference, < 2).
CONCLUSIONS: Biotin can be used to label caprine RBCs. The half-life of RBCs is affected by storage.
CLINICAL RELEVANCE: Blood storage for 28 days significantly reduces the half-life of biotin-labeled RBCs after transfusion.
METHODS: Fifteen 13- to 15-month-old nonlactating goats were randomly assigned to 3 groups (5/group). Each goat served as a donor and recipient and was administered a whole-blood transfusion from a randomly assigned herd mate. The RBCs in whole blood were biotin labeled. Group 1 goats were transfused with fresh biotin-labeled whole blood within 6 hours of collection. Group 2 and 3 goats were transfused with biotin-labeled whole blood stored at 4 °C for 14 and 28 days, respectively. Blood samples were collected at 15 minutes and 1, 2, 3, 5, 7, 14, 21, and 28 days after transfusion. Biotin-labeled RBCs were detected using flow cytometry, and their half-lives were determined using nonlinear regression. The study was conducted from June 10 through September 2, 2025.
RESULTS: Biotin-labeled RBCs were distinctly detected by flow cytometry. The half-lives of biotin-labeled RBCs in goats in groups 1, 2, and 3 were 4.6, 4.3, and 2.5 days, respectively. The half-lives of biotin-labeled RBCs in groups 1 and 2 were longer than in group 3 (Akaike information criterion difference, > 2). There was no difference in biotin-labeled RBC half-lives between groups 1 and 2 (Akaike information criterion difference, < 2).
CONCLUSIONS: Biotin can be used to label caprine RBCs. The half-life of RBCs is affected by storage.
CLINICAL RELEVANCE: Blood storage for 28 days significantly reduces the half-life of biotin-labeled RBCs after transfusion.