Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2003
[Using two-dimensional gel electrophoresis to analyze the regeneration microenvironment of facial nerve and its response to acupuncture].
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Yang Hui, Yang Cairong, Lu Xiaofeng, Liang Chuanyu, Gao Bingqing
Аннотация
OBJECTIVE: To investigate the distribution of proteins in the regeneration microenvironment of facial nerve and its response to acupuncture.
METHODS: We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two-dimensional gel electrophoresis.
RESULTS: We found that most proteins in the regeneration fluid were under relative molecular mass 80 x 10(3) and in the range of pI 4-7. An average of 800 +/- 53 protein spots was found in the control group compared to the 850 +/- 78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups.
CONCLUSION: There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve.
METHODS: We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two-dimensional gel electrophoresis.
RESULTS: We found that most proteins in the regeneration fluid were under relative molecular mass 80 x 10(3) and in the range of pI 4-7. An average of 800 +/- 53 protein spots was found in the control group compared to the 850 +/- 78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups.
CONCLUSION: There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve.