Fertility and sterility · 2006
Preimplantation genetic diagnosis of Marfan syndrome using multiple displacement amplification.
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Lledó Belén, Ten Jorge, Galán Francisco M, Bernabeu Rafael
Аннотация
OBJECTIVE: To evaluate the use of multiple displacement amplification (MDA) for whole-genome amplification in the preimplantation genetic diagnosis (PGD) of Marfan syndrome.
DESIGN: Multiple displacement amplification was used to amplify the whole-genome directly from a single cell. The MDA product was used for polymerase chain reaction (PCR) analysis of five different loci. At this point MDA was used to develop a PGD-Marfan syndrome program.
SETTING: Fertility and gynecology private center in Alicante, Spain.
PATIENT(S): A couple in which the husband is affected by Marfan syndrome and carries a novel mutation in the FBN-1 gene.
INTERVENTION(S): The MDA of single cells and PCR tests for PGD.
MAIN OUTCOME MEASURE(S): Allele drop-out (ADO), amplification efficiency rates, and the ability to detect Marfan syndrome using MDA.
RESULT(S): We report that isothermal whole-genome amplification from single cells allowed analysis of five different loci using standard conditions. The development of a MDA-PGD protocol for Marfan syndrome allowed for the diagnosis of seven embryos. These were biopsied on day 3 of culture and analyzed. Two healthy embryos were transferred 48 hours after culture, resulting in a singleton ongoing pregnancy and the birth of a healthy child.
CONCLUSION(S): The MDA technique is useful for overcoming the problem of insufficient genomic DNA in PGD. The use of MDA as a universal step marks a new cycle for PGD as it allows for the diagnosis of any known gene defect by standard methods and conditions.
DESIGN: Multiple displacement amplification was used to amplify the whole-genome directly from a single cell. The MDA product was used for polymerase chain reaction (PCR) analysis of five different loci. At this point MDA was used to develop a PGD-Marfan syndrome program.
SETTING: Fertility and gynecology private center in Alicante, Spain.
PATIENT(S): A couple in which the husband is affected by Marfan syndrome and carries a novel mutation in the FBN-1 gene.
INTERVENTION(S): The MDA of single cells and PCR tests for PGD.
MAIN OUTCOME MEASURE(S): Allele drop-out (ADO), amplification efficiency rates, and the ability to detect Marfan syndrome using MDA.
RESULT(S): We report that isothermal whole-genome amplification from single cells allowed analysis of five different loci using standard conditions. The development of a MDA-PGD protocol for Marfan syndrome allowed for the diagnosis of seven embryos. These were biopsied on day 3 of culture and analyzed. Two healthy embryos were transferred 48 hours after culture, resulting in a singleton ongoing pregnancy and the birth of a healthy child.
CONCLUSION(S): The MDA technique is useful for overcoming the problem of insufficient genomic DNA in PGD. The use of MDA as a universal step marks a new cycle for PGD as it allows for the diagnosis of any known gene defect by standard methods and conditions.