Acta oto-laryngologica · 2003
Growth associated protein-43 mRNA expression in nucleus ambiguus motoneurons after recurrent laryngeal nerve injury in the rat.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Uno Toshiyuki, Shogaki Kazuhiro, Bamba Hitoshi, Koike Shinobu, Naruse Yoshihisa, Ijima Norio, Tanaka Masaki, Hisa Yasuo
Аннотация
OBJECTIVE: To investigate the time-dependent differences in growth associated protein-43 (GAP-43) mRNA expression in the nucleus ambiguus (NA) motoneurons after recurrent laryngeal nerve (RLN) transection in the rat.
MATERIAL AND METHODS: Animals were sacrificed on Days 1, 7, 14 and 21 after axotomy. The sections were then processed for in situ hybridization of GAP-43 mRNA.
RESULTS: GAP-43 mRNA transiently increased after axotomy, reaching a peak on postoperative Day 7, and then decreased gradually at 14 and 21 days after axotomy. The peak period of GAP-43 expression in the NA is different from those in the facial and hypoglossal nuclei after axotomy.
CONCLUSION: We have already reported that neuronal nitric oxide synthase (nNOS) is induced in the NA motoneurons after axotomy and reaches a peak on postoperative Day 14. This study shows that the expressions of GAP-43 and nNOS are chronologically different.
MATERIAL AND METHODS: Animals were sacrificed on Days 1, 7, 14 and 21 after axotomy. The sections were then processed for in situ hybridization of GAP-43 mRNA.
RESULTS: GAP-43 mRNA transiently increased after axotomy, reaching a peak on postoperative Day 7, and then decreased gradually at 14 and 21 days after axotomy. The peak period of GAP-43 expression in the NA is different from those in the facial and hypoglossal nuclei after axotomy.
CONCLUSION: We have already reported that neuronal nitric oxide synthase (nNOS) is induced in the NA motoneurons after axotomy and reaches a peak on postoperative Day 14. This study shows that the expressions of GAP-43 and nNOS are chronologically different.
Предметные рубрики MeSH
AnimalsAxotomyBase SequenceDisease Models, AnimalGAP-43 ProteinGene Expression RegulationIn Situ HybridizationMaleMedulla OblongataMolecular Sequence DataMotor NeuronsNerve RegenerationNitric Oxide SynthasePeripheral NervesPolymerase Chain ReactionRNA, MessengerRandom AllocationRatsRats, Sprague-DawleyRecurrent Laryngeal Nerve InjuriesReference ValuesSensitivity and Specificity