Comparative biochemistry and physiology. A, Comparative physiology · 1988
Cholinergic-induced electrolyte transport in rat parotid acini.
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Nauntofte B, Dissing S
Аннотация
Secretory responses of parotid acini occurring within 10 sec following cholinergic stimulation were characterized. 1. Measurement of membrane potentials by means of the fluorescent dye diSC3-(5) revealed a value of approximately -59 mV, which remained unaffected on stimulation. 2. Stimulation caused a rapid net loss of 42K+ that was strongly inhibited by the "maxi" K+-channel inhibitor "charybdotoxin" present in scorpion venom. 3. It was calculated that the number of open "maxi" K+-channels per cell was approximately 40 in the unstimulated state and approximately 3000 in the stimulated state. 4. Stimulation caused a transient decrease in the acinar ATP content. 5. Intracellular pH (pHi) measured by means of the fluorescent dye, BCECF, was dependent upon the presence of extracellular HCO3- as well as Na+. Under physiological conditions pHi was 7.27 and stimulation caused a transient decrease of 0.1 pH units due to HCO3- efflux. The decrease was followed by pHi recovery mediated by a Na+/H+ exchange mechanism.