Cutaneous and ocular toxicology · 2025
Chemopreventive role of Luisia tenuifolia Blume ethanolic whole plant extract in DMBA/croton oil-induced skin tumorigenesis in mice.
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Sethuraman Sakthi Priyadarsini, Ramachandran Kumar Pathangi, Vellapandian Chitra
Аннотация
PURPOSE: This study aimed to investigate the chemopreventive potential of the ethanolic extract of Luisia tenuifolia Blume against skin cancer.
METHODS: The ethanolic whole-plant extract of Luisia tenuifolia was tested for acute and sub-chronic dermal toxicity according to the OECD guidelines 402 and 411. Its chemopreventive efficacy, along with the antioxidant and histopathological changes, was assessed using DMBA/Croton oil-induced skin cancer model in mice.
RESULTS: The toxicity studies indicated that the extract was safe up to 2000 mg/kg body weight. The 90-day repeated application of the extract at 500, 1000, and 2000 mg/kg b.w. showed no mortality or toxicity observations. Topical application of the extract at 200 and 400 mg/kg b.w. applied 10 minutes before DMBA/Croton oil treatment delayed the tumor onset. Importantly, there was a significant decrease in tumor incidence, tumor burden, tumor yield, decreased lipid peroxidation and restored superoxide dismutase, catalase, and glutathione peroxidase levels in extract-treated mice compared with the control. The histopathological observations further substantiated the chemopreventive effect, revealing a marked reduction in neoplastic alterations.
CONCLUSION: The ethanolic extract of Luisia tenuifolia was well tolerated up to 2000 mg/kg b.w. and, at 200 and 400 mg/kg b.w., reduced tumor incidence to 80% and 60%, while 5-fluorouracil showed the higher efficacy (50% tumor incidence). Our findings showed the significant chemopreventive activity by enhancing antioxidant defenses and suppressing skin tumorigenesis in mice, warranting further mechanistic and clinical investigations.
METHODS: The ethanolic whole-plant extract of Luisia tenuifolia was tested for acute and sub-chronic dermal toxicity according to the OECD guidelines 402 and 411. Its chemopreventive efficacy, along with the antioxidant and histopathological changes, was assessed using DMBA/Croton oil-induced skin cancer model in mice.
RESULTS: The toxicity studies indicated that the extract was safe up to 2000 mg/kg body weight. The 90-day repeated application of the extract at 500, 1000, and 2000 mg/kg b.w. showed no mortality or toxicity observations. Topical application of the extract at 200 and 400 mg/kg b.w. applied 10 minutes before DMBA/Croton oil treatment delayed the tumor onset. Importantly, there was a significant decrease in tumor incidence, tumor burden, tumor yield, decreased lipid peroxidation and restored superoxide dismutase, catalase, and glutathione peroxidase levels in extract-treated mice compared with the control. The histopathological observations further substantiated the chemopreventive effect, revealing a marked reduction in neoplastic alterations.
CONCLUSION: The ethanolic extract of Luisia tenuifolia was well tolerated up to 2000 mg/kg b.w. and, at 200 and 400 mg/kg b.w., reduced tumor incidence to 80% and 60%, while 5-fluorouracil showed the higher efficacy (50% tumor incidence). Our findings showed the significant chemopreventive activity by enhancing antioxidant defenses and suppressing skin tumorigenesis in mice, warranting further mechanistic and clinical investigations.