Journal of ethnopharmacology · 2025
Huatanhuoxue decoction alleviates airway inflammation by regulating IL-17A signaling pathway in obese asthmatic mice.
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Sun Yun, Jia Yongrui, Yan Xiaodong, Zhang Qiushi, Li Xiaohong, Yang Zhuya, Wei Danxia, Wu Xiangnong, Mao Zewei, Cao Xue, Tong Xiaoyun, Huang Feng
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Huatanhuoxue Decoction (HTHX) is a traditional Chinese formula consisting of nine herbs. It is used to improve obesity-related asthma symptoms and reduce airway inflammation.
AIM OF THE STUDY: To study HTHX effects on airway inflammation in obese asthmatic mice via the IL-17 A signaling pathway.
MATERIALS AND METHODS: Network pharmacology was used to predict the bioactive ingredients in HTHX. Subsequently, an obese asthma model was established by high-fat diet feeding and exposure to house dust mite. The effects of HTHX on obesity-related asthma progression were investigated using histopathological examinations, airway hyperresponsiveness determinations, and enzyme-linked immunosorbent assays. The mechanism of action of HTHX was confirmed by Western blots, flow cytometry, immunohistochemistry, and immunofluorescence analyses.
RESULTS: HTHX alleviated the development in obese asthma mice by improving the pathological condition of lung tissue, airway hyperresponsiveness, and inflammatory factors. Network pharmacology identified the involvement of the IL-17 signaling pathway. HTHX decreased the production of neutrophils and the expression of NETs in lung tissue. HTHX also reduced group 3 innate lymphoid cells and Th17 cells, which are responsible for producing IL-17 A. The production of IL-17 A-related protein was also suppressed. The results indicate that HTHX inhibited the excessive activation of the IL-17 A signaling pathway.
CONCLUSIONS: HTHX alleviated airway inflammation by regulating the IL-17 A signaling pathway in obese asthmatic mice.
AIM OF THE STUDY: To study HTHX effects on airway inflammation in obese asthmatic mice via the IL-17 A signaling pathway.
MATERIALS AND METHODS: Network pharmacology was used to predict the bioactive ingredients in HTHX. Subsequently, an obese asthma model was established by high-fat diet feeding and exposure to house dust mite. The effects of HTHX on obesity-related asthma progression were investigated using histopathological examinations, airway hyperresponsiveness determinations, and enzyme-linked immunosorbent assays. The mechanism of action of HTHX was confirmed by Western blots, flow cytometry, immunohistochemistry, and immunofluorescence analyses.
RESULTS: HTHX alleviated the development in obese asthma mice by improving the pathological condition of lung tissue, airway hyperresponsiveness, and inflammatory factors. Network pharmacology identified the involvement of the IL-17 signaling pathway. HTHX decreased the production of neutrophils and the expression of NETs in lung tissue. HTHX also reduced group 3 innate lymphoid cells and Th17 cells, which are responsible for producing IL-17 A. The production of IL-17 A-related protein was also suppressed. The results indicate that HTHX inhibited the excessive activation of the IL-17 A signaling pathway.
CONCLUSIONS: HTHX alleviated airway inflammation by regulating the IL-17 A signaling pathway in obese asthmatic mice.