Journal of assisted reproduction and genetics · 1995
Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa.
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Obruca A, Mock K, Feichtinger W, Lunglmayr G
Аннотация
PURPOSE: Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration.
METHODS: Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.
RESULTS: One healthy boy and two girls (twin pregnancy) were born.
CONCLUSIONS: The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.
METHODS: Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.
RESULTS: One healthy boy and two girls (twin pregnancy) were born.
CONCLUSIONS: The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.