ANALYSIS OF PHENOLIC ACIDS AND ANTIBACTERIAL ACTIVITY OF EXTRACTS OBTAINED FROM THE FLOWERING HERBS OF CARD UUS ACANTHOIDES L.

In this work the phenolic acids in crude methanol extracts from the flowering herbs of Carluus acanthoides L. were identified. The samples containing free phenolic acids and those released after acid and alka- line hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Chlorogenic, protocatechuic, p- coumaric, caffeic, syringic, p-hydroxybenzoic, ferulic, vanillic, gentisic and gallic acids were detected in fractions of the methanolic extract obtained from the flow- ering herb of C. acanthoides. This is the first study concerning the qualitative analysis of phenolic compounds and antibacterial activity of fractions and aqueous, ethyl acetate, dichloromethane, acetone and methanol (50, 80 and 100% v/v) extracts of flowering herbs of C. acanthoides L. The antimicrobial activity of tested extracts was determined in vito against reference microorganisms of Gram-positive bacteria, Gram-negative bacteria and fungi belonging to yeasts. The results of this study support the medical usage of C. acanthoides L. due to its antimicrobial properties.