Journal of global antimicrobial resistance · 2018
Draft genome sequence of an extended-spectrum β-lactamase (CTX-M-15)-producing Escherichia coli ST10 isolated from a nasal sample of an abattoir worker in Cameroon.
СтатьяМногоцентровое исследованиеПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Founou Luria Leslie, Founou Raspail Carrel, Allam Mushal, Ismail Arshad, Essack Sabiha Yusuf
Аннотация
OBJECTIVES: Here we report the draft genome sequence of Escherichia coli strain HN503E1II isolated from a nasal sample of an abattoir worker in Cameroon.
METHODS: Bacterial genomic DNA of E. coli HN503E1II was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antibiotic resistance genes, virulence factors, plasmids and the sequence type (ST) were identified.
RESULTS: The genome comprised a circular chromosome of 4 674201bp, with a 50.78% G+C content and several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, sulphonamides, trimethoprim, macrolides and tetracycline. The isolate was assigned to ST10 with 100% identity among the seven housekeeping genes. In silico analysis also revealed the presence six plasmid types and one virulence factor.
CONCLUSION: The genome sequence reported here will provide valuable information for a better understanding of the genetic structure of the E. coli genome in Africa.
METHODS: Bacterial genomic DNA of E. coli HN503E1II was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antibiotic resistance genes, virulence factors, plasmids and the sequence type (ST) were identified.
RESULTS: The genome comprised a circular chromosome of 4 674201bp, with a 50.78% G+C content and several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, sulphonamides, trimethoprim, macrolides and tetracycline. The isolate was assigned to ST10 with 100% identity among the seven housekeeping genes. In silico analysis also revealed the presence six plasmid types and one virulence factor.
CONCLUSION: The genome sequence reported here will provide valuable information for a better understanding of the genetic structure of the E. coli genome in Africa.