Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2004
[Bridging rat sciatic nerve defects with the composite nerve-muscle autografts wrapped with human amnion matrix membrane].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Duan Xian-Lai, Xu Yuan-Zhong, Zeng Zhi-Cheng
Аннотация
OBJECTIVE: To explore the possibility of bridging the sciatic nerve defects with the composite nerve-muscle (NM) autografts wrapped with human amnion matrix membrane (HAMM) in rats.
METHODS: Fifty-four Wistar rats were divided randomly into 3 groups (n = 18), and about 10 mm of right sciatic nerve defects were bridged with the composite NM autografts, the nerve autografts and the denatured skeletal muscle autografts respectively (Group A, B, and C), and all grafts were wrapped with HAMM. After the operation, the regenerated nerves were assessed by Fast blue retrograde tracing, neurofilament (NF) immnohistochemical staining, regenerated axons counting, measuring the diameter and myelin thickness of the regenerated axons, and quantifying the wet weight of tibialis anterior muscle.
RESULTS: In Group A and B, there were more fluorescent-labeled cells in dorsal root ganglion and spinal cord than in Group C. The regenerated NFs were sparse and disordered in Group C, but dense and regular in Group A and B. Group A and Group B showed results superior to Group C as to other histologic and morphologic characters (P < 0.05), whereas there was no significant difference between Group A and B (P > 0.05).
CONCLUSION: The composite NM autografts wrapped with HAMM in this study can well bridge and repair sciatic nerve defects in rats.
METHODS: Fifty-four Wistar rats were divided randomly into 3 groups (n = 18), and about 10 mm of right sciatic nerve defects were bridged with the composite NM autografts, the nerve autografts and the denatured skeletal muscle autografts respectively (Group A, B, and C), and all grafts were wrapped with HAMM. After the operation, the regenerated nerves were assessed by Fast blue retrograde tracing, neurofilament (NF) immnohistochemical staining, regenerated axons counting, measuring the diameter and myelin thickness of the regenerated axons, and quantifying the wet weight of tibialis anterior muscle.
RESULTS: In Group A and B, there were more fluorescent-labeled cells in dorsal root ganglion and spinal cord than in Group C. The regenerated NFs were sparse and disordered in Group C, but dense and regular in Group A and B. Group A and Group B showed results superior to Group C as to other histologic and morphologic characters (P < 0.05), whereas there was no significant difference between Group A and B (P > 0.05).
CONCLUSION: The composite NM autografts wrapped with HAMM in this study can well bridge and repair sciatic nerve defects in rats.