American journal of reproductive immunology (New York, N.Y. : 1989) · 2013
Cutting edge: IL-10-producing regulatory B cells in early human pregnancy.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Rolle Luise, Memarzadeh Tehran Maryam, Morell-García Anselm, Raeva Yanitsa, Schumacher Anne, Hartig Roland, Costa Serban-Dan, Jensen Federico, Zenclussen Ana Claudia
Аннотация
PROBLEM: The function of IL-10 producing regulatory B cells (Breg) during gestation is unknown. Here, we aimed to understand their participation in early pregnancy.
METHOD: CD19(+) CD24(hi) CD27(+) B cell frequency, measured by flow cytometry, increased with pregnancy onset but not in the case of spontaneous abortions.
RESULTS: B cells from non-pregnant women cultured with serum from normal pregnant women produced higher IL-10 levels than those cultured with serum from spontaneous abortion patients or autologous serum. CD19(+) -activated B cells from pregnant women strongly suppressed TNF-a production by CD4(+) T cells when cocultured. We identified hCG as an important factor regulating the number and function of Breg during pregnancy.
CONCLUSIONS: Breg emerge as important players in pregnancy; they suppress undesired immune responses from maternal T cells and are therefore important for tolerance acquisition.
METHOD: CD19(+) CD24(hi) CD27(+) B cell frequency, measured by flow cytometry, increased with pregnancy onset but not in the case of spontaneous abortions.
RESULTS: B cells from non-pregnant women cultured with serum from normal pregnant women produced higher IL-10 levels than those cultured with serum from spontaneous abortion patients or autologous serum. CD19(+) -activated B cells from pregnant women strongly suppressed TNF-a production by CD4(+) T cells when cocultured. We identified hCG as an important factor regulating the number and function of Breg during pregnancy.
CONCLUSIONS: Breg emerge as important players in pregnancy; they suppress undesired immune responses from maternal T cells and are therefore important for tolerance acquisition.