Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae · 2007
[Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Huang Jin, Liu Ping, Chen Yong-Jian, Zhang Hong, Zheng Dan-Xia, Zhang Xiao-Wei, Qiao Jie
Аннотация
OBJECTIVE: To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).
METHOD: Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.
RESULT: The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.
CONCLUSION: Single cell fluorescent PCR is a stable and reliable approach for the PGD.
METHOD: Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.
RESULT: The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.
CONCLUSION: Single cell fluorescent PCR is a stable and reliable approach for the PGD.