[Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction].

OBJECTIVE

To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).

METHOD: Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.

RESULT: The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.

CONCLUSION

Single cell fluorescent PCR is a stable and reliable approach for the PGD.