[The observation on infection of trasgenic mice (PVR Tg21) with polioviruses type 3].

OBJECTIVE

To test and verify whether PVR Tg21 transgenic mice can be used as an animal model or not.

METHODS

PVR Tg21 transgenic mice were infected with 3 poliovirus strains with different neurovirulence intraspinally.

RESULTS

All the mice showed clinical paralysis with characteristic lesions in neurons. The symptoms varied with different potency of virus. The onset of clinical paralysis time for inoculated mice were approximately 2 days with Leon virus, 5 days with Sabin strain, and 4 days with Vaccine-321. More than 90% of mice inoculated with Leon strain died within 3 days after clinical paralysis and only 35% mice inoculated with Sabin-3 died at average of 6 days, and the PD50 are 10(1.1), 10(2.6), and 10(4.97) TCID50 and LD50 are 10(1.1), 10(3.33) and 10(5.33) TCID50, respectively. Different virus inoculated intraspinally caused different histopathological lesions.

CONCLUSIONS

The PVR Tg21 transgenic mice could be a new animal model for neurovirulent evaluation of poliovaccines and epidemiological surveillance.