Journal of advanced research · 2025
Supra-Carbon dots for methyl-paraoxon detection using a new controllable IFE-based method.
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Luo Mai, Cai Jihai, Pu Sijia, Wang Ting, Wu Sijia, Wang Mengwei, Wang Shuangpeng, Qu Songnan, Zou Liang, Li Peng, Wei Jinchao
Аннотация
INTRODUCTION: Pesticide residues pose a significant threat to human health. Inner filter effect (IFE) stands out for its ability to enable the rapid and cost-effective establishment of fluorescent detection systems. Nevertheless, the practical implementation is often hindered by issues such as low spectral overlap and interference from impurities, which can compromise its effectiveness.
OBJECTIVES: To develop novel AIEE-type CDs and apply them to improve the efficiency of IFE-based strategies. With the aid of AIEE-type CDs, we aim to develop a multi-channel detection platform that provides more reliable fluorescent probes for real samples and imaging applications.
METHODS: Preparation of N, S-CDs was achieved using a novel combination of sources. The activation mechanism of Supra-CDs' AIEE characteristics was verified. In the methodological experiments, detection performance of Supra-CDs was tested to confirm their improvement in the IFE-based strategy, practical applicability for real sample detection, and imaging capability.
RESULTS: In this work, p-PA and thiourea were used for the first time to prepare N, S-CDs. After adding water, the fluorescence intensity of Supra-CDs increased by nearly 360 % compared to N, S-CDs. Spectral overlap rates of the target and probe in the controllable IFE-based system improved from 1.80 % to 33.11 %, indicating superior analytical performance in aqueous solutions compared to ethanol. The fluorescent method exhibited a linear range of 0.5-100 μM, with a detection limit of 0.5 μM. Furthermore, a smartphone-based system has been proposed for the colorimetric analysis of methyl paraoxon, offering a more convenient and efficient alternative. Additionally, Supra-CD demonstrated exceptional capabilities in cell imaging, specifically targeting intracellular lipids within HepG2 cells.
CONCLUSION: This study unveils a new type of Supra-CDs that exhibit AIEE property. It also introduces a creative fluorescent sensor with tunable IFE. In summary, this research delivers important insights into AIEE-type Supra-CDs and affirms their potential to inspire broader applications.
OBJECTIVES: To develop novel AIEE-type CDs and apply them to improve the efficiency of IFE-based strategies. With the aid of AIEE-type CDs, we aim to develop a multi-channel detection platform that provides more reliable fluorescent probes for real samples and imaging applications.
METHODS: Preparation of N, S-CDs was achieved using a novel combination of sources. The activation mechanism of Supra-CDs' AIEE characteristics was verified. In the methodological experiments, detection performance of Supra-CDs was tested to confirm their improvement in the IFE-based strategy, practical applicability for real sample detection, and imaging capability.
RESULTS: In this work, p-PA and thiourea were used for the first time to prepare N, S-CDs. After adding water, the fluorescence intensity of Supra-CDs increased by nearly 360 % compared to N, S-CDs. Spectral overlap rates of the target and probe in the controllable IFE-based system improved from 1.80 % to 33.11 %, indicating superior analytical performance in aqueous solutions compared to ethanol. The fluorescent method exhibited a linear range of 0.5-100 μM, with a detection limit of 0.5 μM. Furthermore, a smartphone-based system has been proposed for the colorimetric analysis of methyl paraoxon, offering a more convenient and efficient alternative. Additionally, Supra-CD demonstrated exceptional capabilities in cell imaging, specifically targeting intracellular lipids within HepG2 cells.
CONCLUSION: This study unveils a new type of Supra-CDs that exhibit AIEE property. It also introduces a creative fluorescent sensor with tunable IFE. In summary, this research delivers important insights into AIEE-type Supra-CDs and affirms their potential to inspire broader applications.