[Effect of different expression levels of GRIM-19 on the resistance of prostate cancer cells to docetaxel chemotherapy].

OBJECTIVE

To investigate the effect of GRIM-19 on the resistance of carcinoma cells to the chemotherapeutic agent docetaxel in the treatment of PCa.

METHODS

Using siRNA technology to interfere with the gene expression in PCa cells, we established a model of GRIM-19 overexpression / knockdown in PCa cells. We investigated the effect of different expression levels of GRIM-19 on docetaxel-induced death of the PCa cells by qPCR, Western blot and flow cytometry, and assessed the value of GRIM-19 in reducing the chemotherapy-resistance of PCa cells.

RESULTS

GRIM-19 was down-regulated in PCa tissues and cells. Knockout of GRIM-19 significantly decreased the expression of siGRIM19 in the PC-3 and LNCaP cells, and reduced their death rate when treated with docetaxel compared with the control group. The expressions of GRIM-19 mRNA and protein were remarkably upregulated after transfection with GRIM-19, and the overexpressed GRIM-19 promoted the death of the PC-3 and LNCaP cells treated with docetaxel in a dose-dependent manner. Flow cytometry analysis showed a lower apoptosis rate of PC-3-R cells than that of PC-3 cells at different time points of docetaxel-induction at different doses.

CONCLUSION

GRIM-19 is a PCa suppressor gene with a significant facilitating effect on the apoptosis of PCa cells, and the overexpression of GRIM-19 promotes docetaxel-induced PCa cell death and improves the sensitivity of chemotherapy.