Pre-formed donor-specific alloantibodies (DSA) detected only by luminex technology using HLA-coated microspheres and causing acute humoral rejection and kidney graft dysfunction.

The different methodologies for the detection of HLA sensitization could have discrepant results. At the moment, luminex technology seems--in our opinion--to be the most sensitive and safest method for antibody detection, and it should be taken into account during the transplantation process and as a means of indicating immunossuppresive regimen modulation.