[Combined effects of sub-chronic exposure to lead and cadmium on physiological and biochemical indexes of blood in SD rats].

OBJECTIVE

The combined subchronic effects of exposure to lead acetate [Pb (NO3)2] and cadmium chloride [CdCl2 x 2.5H2O] on blood physiological and biochemical indexes of rats were detected to investigate the hazards of environmentally relevant, low-dose exposure to these compounds.

METHODS

80 SD rats were randomly divided into three experiment groups and one control group. The rats in the three experiment groups were orally administrated with Pb(NO3)2 and CdCl2 x 2.5H2O combined solution at the doses of 29.96, 89.88 and 269.65 mg/kg for 90 days respectively, and the rats in control group were orally administrated with water. Blood were collected every 30 days to determine physiological and biochemical indexes.

RESULTS

In each poisoning groups, WBC, RBC and HGB increased during early experiment period and then decreased. ALT, AST and BU increased all the experiment time. GLU decreased in the experiment time. Compared with control group, TC increase at high-dose poisoning group and TG decrease at low-dose poisoning group. The TP, ALB, GLO and CRE in the poisoning groups were not significantly different from those in the control group. And the hepatic cells and renal tubule epithelial cells showed granular degeneration, vacuolar degeneration and necrosis in poisoning groups.

CONCLUSION

Low-dose Pd-Cd combined exposure could significantly change physiological and biochemical indexes of blood and cause hepatic and renal pathological injury of SD rats.