Occurrence and distribution of trace levels of antibiotics in surface waters and soils driven by non-point source pollution and anthropogenic pressure.

Antibiotics in surface waters and soils are growing public health concerns and treated wastewater has often been identified as the main source of antibiotics. However, few studies have been conducted to evaluate the occurrence and concentrations of antibiotics in coastal cities without direct impact of wastewater discharge. In this study, the occurrence of 14 antibiotics including four macrolides, three sulfonamides, three β-lactams, lincomycin, chloramphenicol, furazolidon, and monensin in surface waters and soils in Singapore were analyzed with SPE-LC-ESI-MS/MS. The detected concentrations of antibiotics were all below 82.5 ng/L in surface waters and below 80.6 ng/g dry wt in soils. These concentrations were significantly lower than other cities that were under the impact of treated wastewater discharge, suggesting that reduction of treated wastewater discharge reduces occurrence of antibiotics in the environment. However, the wide occurrence of trace levels of antibiotics suggest that other factors may have contributed to detected environmental antibiotics. Population density was positively correlated with concentrations of clarithromycin, lincomycin, azithromycin, and sulfamethoxazole in surface waters, suggesting that non-point source pollution due to anthropogenic pressure may contribute to the wide detection of trace levels of antibiotics. The potential impact of antibiotic use, natural production, and half-lives of antibiotics were further discussed. Further studies are needed to evaluate how anthropogenic activities other than wastewater discharge may contribute to the occurrence of trace level antibiotics and their associated health risks in urban environments.