[Expression and significance of IL-10 in human chorionic villi of recurrent spontaneous abortion].

AIM: To investigate the correlation between interleukin-10(IL-10) and recurrent spontaneous abortion as well as the significance of them through researching the comparison of histological localization of IL-10, expression comparison of IL-10 mRNA in human chorionic villi of pregnant women with recurrent spontaneous abortion and normal abortion.

METHODS

To detect expression comparison of IL-10 mRNA and histological localization of IL-10 in human chorionic villi of 35 pregnant women with recurrent spontaneous abortion(experimental group) and 35 normal abortion women(control group) by reverse transcription-polymerase chain reaction and immunohistochemistry. To analysis expression of IL-10 in serum of the two groups by ELISA.

RESULTS

The detection of immunohistochemistry showed that there are expressions of IL-10 both in the two groups, the expressions of IL-10 in the experimental group were lower than the control group(P<0.05).The results of the RT-PCR showed that the expressions of IL-10 of experimental group were lower than the control group(P<0.05). ELISA analysis showed that the expressions of IL-10 of the experimental group were lower than the control group(P<0.05).

CONCLUSION

The results of detection of immunohistochemistry and the expression of mRNA of IL-10 in human chorionic villi as well as its expressions in serum of experimental group were lower than the control group. IL-10 would play an important role in maintaining normal pregnancy.