[Study on temperature measurement and control for microfluidic systems].

A technique for temperature control and measurement in microfluidic systems was developed. The technique is easy to be implemented in an analytical lab and the experimental setup is relatively simple and inexpensive. The parts for temperature measurement include a CCD camera, a fluorescence microscope and an image acquisition card. The spatial distribution and the temporal variation of temperature in microfluids were measured. The method is non-invasive and the spatial and temporal resolutions were 0. 8 microm and 40 ms, respectively a thermal control device was built using indium-tin-oxide (ITO) coated glass as heater, taking advantage of its transparent quality. The temperature could be controlled with a precision of +/-0.1 degrees C. The image processing procedure was optimized and the efficacy of the method was demonstrated.