The application of CdSe quantum dots with multicolor emission as fluorescent probes for cell labeling.

Herein, highly luminescent CdSe quantum dots (QDs) with emissions from the blue to the red region of visible light were synthesized by using a simple method. The emission range of the CdSe QDs could be tuned from λ=503 to 606 nm by controlling the size of the CdSe QDs. Two amino acids, L-tryptophan (L-Trp) and L-arginine (L-Arg), were used as coating agents. The quantum yield (QY) of CdSe QDs (green color) with an optimized thickness could reach up to 52 %. The structures and compositions of QDs were examined by using X-ray diffraction (XRD) and transmission electron microscopy (TEM). Optical properties were studied by using UV/Vis and photoluminescence (PL) spectroscopy and a comparison was made between uncoated and coated CdSe QDs. The amino acid-modified β-cyclodextrin (CD)-coated CdSe QDs presented lower cytotoxicity to cells for 48 h. Furthermore, amino acid-modified β-CD-coated green CdSe QDs in HepG2 cells were assessed by using confocal laser scanning fluorescence microscopy. The results showed that amino acid-modified β-CD-coated green CdSe QDs could enter tumor cells efficiently and indicated that biomolecule-coated QDs could be used as a potential fluorescent probe.