Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. I. Nucleotide sequence of the nucleoprotein gene.

The complete nucleotide sequence of cloned cDNAs corresponding to the full-length mRNA encoding the NP protein of the Yamagata-1 strain of subacute sclerosing panencephalitis (SSPE) virus was determined. The gene is composed of 1683 nucleotides and contains a single large open reading frame, which is capable of encoding 525 amino acids with a molecular weight of 58,399. Comparison of the nucleotide and predicted amino acid sequences with those of the Edmonston strain of measles virus (MV) showed that the gene and the protein were highly conserved. However, the antigenic sites on the NP protein of the Yamagata-1 strain were found to be changed by an epitope analysis using monoclonal antibodies against the NP protein of MV. Only 1 of 4 monoclonal antibodies reacted with the NP protein of SSPE virus, and the other three antibodies did not. Almost identical changes in nucleotides and amino acids were found to occur in the NP gene of the Yamagata-1 strain when compared with the IP-3-Ca strain of another SSPE virus. In addition, the deduced secondary structure of the NP protein of the IP-3-Ca strain was similar to that of the Yamagata-1 strain, but differed from the MV. These results suggest that the NP proteins of SSPE viruses have a common property that is different from MV.