Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2014
[Ketamine induces apoptosis of human uroepithelial SV-HUC-1 cells].
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Huang Liang, Tang Zhengyan, Li Dongjie, Wang Guilin, Huang Ke, Zhou Jie, Li Yonglin, Gao Benmin, Chen Junjie
Аннотация
OBJECTIVE: To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SVHUC-1) and the pathogenesis of ketamine-associated cystitis.
METHODS: SV-HUC-1 cells were cultured under various concentrations of ketamine and differenttime. Flow cytometry was used to analyze the rate of cell apoptosis. The protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot.
RESULTS: Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. The expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was significantly higher. The protein level of pro-caspase-3 was significantly lower, and that of cleaved caspase-3 was significantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05).
CONCLUSION: Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.
METHODS: SV-HUC-1 cells were cultured under various concentrations of ketamine and differenttime. Flow cytometry was used to analyze the rate of cell apoptosis. The protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot.
RESULTS: Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. The expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was significantly higher. The protein level of pro-caspase-3 was significantly lower, and that of cleaved caspase-3 was significantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05).
CONCLUSION: Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.