Purification, crystallization and preliminary X-ray diffraction analysis of haemorrhagin IV from the snake venom of Agkistrodon acutus.

Haemorrhagin IV, a medium molecular weight haemorrhagin from the snake venom of Agkistrodon acutus (AaHIV), has been purified and crystallized. The molecular weight and isoelectric point of AaHIV are 44 kDa and pI 5.0, respectively. The crystal belongs to space group C222(1) with unit-cell dimensions of a = 124.2, b = 114.5, c = 98.4 A, and could o diffract X-rays to 3.0 A, resolution. There are one or two molecules in the crystallographic asymmetric unit.