Identification of a novel long non-coding RNA (LOC105377284) regulating PPARγ-dependent adipogenesis.

BACKGROUND

Long non-coding RNAs (lncRNAs) are key modulators of gene expression and play critical roles in metabolic disorders. This study aimed to investigate the expression of LOC105377284, a human putative homolog of the murine lnc-U90926, across obesity classes and evaluate its correlation with PPARγ, a master regulator of adipogenesis and glucose metabolism.

METHODS

Sixty-seven participants were dividedinto four BMI-defined groups: underweight/normal (18.5–24.9 kg/m²; n = 17), overweight (25–29.9 kg/m²; n = 16), obesity class I (30–39.99 kg/m²; n = 20), and obesity class II/III (> 40 kg/m²; n = 14). PPARγ and LOC105377284 transcript levels were measured using quantitative real-time PCR (qRT-PCR).

RESULTS

The expression levels of both PPARγ and LOC105377284 genes were positively correlated across obesity classes. In particular, their expression was significantly elevated in individuals with morbid obesity (BMI ≥ 40). The correlations between the LOC105377284 gene and blood parameters known to be affected by the PPARγ gene (total cholesterol, HDL-C, LDL-C, triglycerides, vitamin B12, vitamin D, ferritin, eosinophils, neutrophils, fasting blood glucose) were investigated. While PPARG is a well-established regulator in the development and metabolism of fat cells, LOC105377284, a potential novel biomarker or regulatory factor, may play a role in obesity-related metabolic changes.

CONCLUSIONS

This study demonstrates that LOC105377284 expression, measured in peripheral blood, is correlated with obesity classification and shows a significant association with PPARγ expression; however, further functional studies are required to elucidate its biological role.