Cytotoxic effect of a cyclic nonapeptide from Agrostemma githago L. against non-small cell lung carcinoma H460 cells.

One new cyclic nonapeptide, named agroscyclopeptide A (1), was isolated from Agrostemma githago L. The planar structure of agroscyclopeptide A was determined using spectroscopic techniques and MS analyses. The amino acids absolute configurations were identified by advanced Marfey's method. The potential anti-lung-cancer activity of compound 1 was evaluated using CCK-8 assay, through which its cytotoxic effects on NCl-H460 cells were analysed by assessing cellular viability. The results revealed that the compound 1 exhibited dose-dependent inhibition of NCL-H460 cells with a calculated IC50 value of 4.6  μM, demonstrating efficacy comparable to that of cisplatin under identical experimental condition.