Toxicological analysis of whole blood samples by means of Bond-Elut Certify columns and gas chromatography with nitrogen-phosphorus detection.

The application of Bond-Elut Certify solid-phase extraction columns to the systematic toxicological analysis of whole blood was evaluated. The reproducibility of the extraction was tested with thirteen drugs varying in physico-chemical properties. Analysis was performed with capillary gas chromatography with nitrogen-selective detection. The recoveries were reproducible, as long as other limiting factors, e.g., chromatographic behaviour or volatility, do not play a significant role. The effect of limiting chromatographic behaviour was studied in more detail with the more sensitive mass spectrometry with selected-ion monitoring after converting the extracted morphine into its ditrimethylsilyl derivative.