[Experimental studies on microcirculation of the hindlimb after ischemia and reperfusion in the rabbits. 1. Establishment of an in vivo method for observing microcirculation using tendon surface microvessels on the dorsum of the foot].

The blood supply system of the extensor digitorum longus tendon in the rabbit hindlimb is observed by the aid of dye injection technique and dissecting microscope. We find that the segment proximal to metatarsophalangeal joint of this tendon is an ideal site for observation of in vivo microcirculation. In this site, there are abundant microvessels on the surface of the tendon, and as red microvessels are on white tendon, it forms a sharp contrast. In addition, the tendon is only covered by skin and little loose connective tissue, so it is easy to expose. On the bases of abovementioned findings, a new in vivo method for observing microcirculation in the rabbit hindlimb is developed. In our experiences, the advantages of this method are as follows: (1) It is simple and convenient; (2) The time required for preparation is short (about 10-15 min), and the observation period can be extended (over 6 h.); (3) Surgical trauma is not serious; (4) The image is clear. This method opens up a new way to using rabbit hindlimb ischemia model to study microcirculation changes in vivo following reperfusion after extremity ischemia.