Effect of aflatoxin B1 on some liver microsomal enzymes in mice fed cyanide supplemented diets.

Aniline hydroxylase, glucose-6-phosphatase, NADPH- and NADH-cytochrome C reductase activities were measured in liver microsomes prepared from four groups of female mice. Mice were fed either control diets alone or KCN (0.357, microgram/kg body wt/day) supplemented diets or control diets plus AFB1 (0.35 microgram/kg body wt/day) administration (ip) on the 8, 9 and 10th day or the KCN supplemented diet plus AFB, administration (ip) on the 8, 9 and 10th day. KCN and AFB1 consistently elevated the activities of the enzymes. Simultaneous administration of both toxins potentiated their effects on the enzymes with the exception of glucose-6-phosphatase. Increases in microsomal protein/liver wt ratios, liver wt/body wt ratios and these enzyme activities were probably indicative of microsomal enzyme induction.