Crystallization and preliminary X-ray analysis of a thermostable pectate lyase PL 47 from Bacillus sp. TS 47.

The thermostable pectate lyase PL 47 from Bacillus sp. TS 47, with a molecular weight of 50 kDa, was crystallized by the hanging-drop vapour-diffusion method using 2-propanol and polyethylene glycol 4000 as precipitants. The crystals belong to the trigonal space group P3(1)21, with unit-cell parameters a = b = 58.8, c = 229.7 A, gamma = 120 degrees. The calculated V(M) based on one molecule per asymmetric unit is 2.30 A(3) Da(-1). A native data set from a frozen crystal was collected to 1.8 A resolution using synchrotron radiation at SPring-8. A molecular-replacement solution was obtained using the structure of pectate lyase from B. subtilis as a model.