[Recognition and quantitative contrast characteristic components for root of Chinese angelica].

A method of multi-component quantitative analysis in root of Chinese Angelica has been developed. The analytical mode was reversed-phase HPLC with photodiode array detector. Based on comparison of UV spectra, a software of UV spectra database to assist recognition has been established. The solvent system, a gradient program of mobile phase with 2-propanol/acetonitrile/water can separate a lot of liposoluble components in root of Chinese Angelica. UV detection wavelength was set at 202 nm and the flow rate was 1 mL/min on a Hypersil ODS column. Twenty-three components in two roots of Chinese Angelica were recognized using characteristic parameters of chromatography and UV spectroscopy. It is the base of quantitative contrast to recognize many characteristic components without standard components in root of Chinese Angelica. For two samples of root of Chinese Angelica both have 23 characteristic components and the concentrations of some components were similar and those of others were different. The method is convenient and reproducible. It can be used for the quality control of the root of Chinese Angelica without the use of standards.