Memorias do Instituto Oswaldo Cruz · 2026
Investigation of neurotropic arboviruses in wild and domestic animals in Amazon region, 2023-2024.
СтатьяНазвание и аннотация: Перевод в очереди
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Hayashi Daniel Jun, Nunes Bruno Tardelli Diniz, Silva Sandro Patroca da, Cunha Tânia Cristina Alves da Silveira da, Paiva Francisco Amilton Dos Santos, Prazeres Ivy Tsuya Essashika, Santos Alessandra da Conceição Miranda, Barros Landeson Junior Leopoldino, Ribeiro Ana Cláudia da Silva, Sobral Felipe Baraldi, Fernandes Luiz Mário, Coelho Taciana Fernandes Souza Barbosa, Carvalho Valéria Lima, Cruz Ana Cecilia Ribeiro, Medeiros Daniele Barbosa de Almeida, Casseb Livia Medeiros Neves
Аннотация
BACKGROUND: Arboviruses represent a potential threat to global public health due to their ability to infect various vertebrate hosts and vectors, as well as their adaptability to diverse ecosystems, allowing them to expand geographically across continents.
OBJECTIVE: The present study aimed to describe the molecular epidemiology of encephalitic arboviruses, including Venezuelan equine encephalitis virus (VEEV), Eastern equine encephalitis virus (EEEV), Western equine encephalitis virus (WEEV), West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Toscana virus (TOSV), and Rift Valley fever virus (RVFV) in nervous tissue samples from domestic and wild animals from the Northern region of Brazil, between February 2023 and June 2024.
METHODS: Samples negative for rabies virus were analysed by reverse transcription real-time polymerase chain reaction (RT-qPCR) targeting Alphavirus, Orthoflavivirus and Phlebovirus species.Positive samples were subjected to viral isolation in cell culture and whole-genome sequencing using next-generation sequencing.Phylogenetic and molecular clock analyses were performed to characterise viral lineages and evolutionary relationships.
FINDINGS: Two samples tested positive for arboviruses by RT-qPCR: one SLEV sample from the state of Amazonas, which showed a low viral load, preventing virus isolation and sequencing, and one Madariaga virus (MADV) sample from the state of Pará, which could be isolated and sequenced.The isolated agent belongs to lineage III of EEEV, showing most similarity to strains from Guyana and Argentina.
MAIN CONCLUSIONS: The present study detected two arboviruses in animals, suggesting its circulation in the study area.
OBJECTIVE: The present study aimed to describe the molecular epidemiology of encephalitic arboviruses, including Venezuelan equine encephalitis virus (VEEV), Eastern equine encephalitis virus (EEEV), Western equine encephalitis virus (WEEV), West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Toscana virus (TOSV), and Rift Valley fever virus (RVFV) in nervous tissue samples from domestic and wild animals from the Northern region of Brazil, between February 2023 and June 2024.
METHODS: Samples negative for rabies virus were analysed by reverse transcription real-time polymerase chain reaction (RT-qPCR) targeting Alphavirus, Orthoflavivirus and Phlebovirus species.Positive samples were subjected to viral isolation in cell culture and whole-genome sequencing using next-generation sequencing.Phylogenetic and molecular clock analyses were performed to characterise viral lineages and evolutionary relationships.
FINDINGS: Two samples tested positive for arboviruses by RT-qPCR: one SLEV sample from the state of Amazonas, which showed a low viral load, preventing virus isolation and sequencing, and one Madariaga virus (MADV) sample from the state of Pará, which could be isolated and sequenced.The isolated agent belongs to lineage III of EEEV, showing most similarity to strains from Guyana and Argentina.
MAIN CONCLUSIONS: The present study detected two arboviruses in animals, suggesting its circulation in the study area.