Anticancer research · 2010
Histological detection of minimal metastatic disease in inguinal non-sentinel lymph nodes in penile cancer.
СтатьяМногоцентровое исследованиеНазвание и аннотация: Перевод в очереди
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Naumann Carsten Maik, Macquarrie Ashley, Van Der Horst Christof, Hamann Moritz Franz, Al-Najar Amr, Kaufmann Sascha, Hegele Axel, Korda Joanna Beate, Bolenz Christian, Jochens Arne, Jünemann Klaus-Peter, Leuschner Ivo
Аннотация
UNLABELLED: The aim of this study was to detect lymphatic spread by serial step-section technique in non-sentinel lymph nodes (NSLNs), which were earlier assessed as negative by histological examination.
PATIENTS AND METHODS: Inguinal dissection specimens of 13 men with penile cancer were investigated. The LNs were sectioned at multiple levels (150 mum-intervals) and then H&E- and immuno-stained for cytokeratin (Lu-5).
RESULTS: 196 LNs of 13 men were examined. In 2 out of 13 patients (15%) previously ranked as pN0, minimal lymph node involvement was detected by serial step sections and both immunohistochemistry and H&E staining. Both patients have had an uneventful follow-up of currently 62 and 16 months.
CONCLUSION: Conventional histological examination of NSLNs fails to detect lymphatic spread in penile cancer. Step-section technique at 3 section levels, rather than immunohistochemistry, helps to safely detect minimal metastatic disease. The prognostic relevance is still unclear and has to be investigated in larger cohort studies.
PATIENTS AND METHODS: Inguinal dissection specimens of 13 men with penile cancer were investigated. The LNs were sectioned at multiple levels (150 mum-intervals) and then H&E- and immuno-stained for cytokeratin (Lu-5).
RESULTS: 196 LNs of 13 men were examined. In 2 out of 13 patients (15%) previously ranked as pN0, minimal lymph node involvement was detected by serial step sections and both immunohistochemistry and H&E staining. Both patients have had an uneventful follow-up of currently 62 and 16 months.
CONCLUSION: Conventional histological examination of NSLNs fails to detect lymphatic spread in penile cancer. Step-section technique at 3 section levels, rather than immunohistochemistry, helps to safely detect minimal metastatic disease. The prognostic relevance is still unclear and has to be investigated in larger cohort studies.