Animal reproduction science · 2007
In vitro production of llama (Lama glama) embryos by IVF and ICSI with fresh semen.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Conde Pamela A, Herrera Carolina, Trasorras Virginia L, Giuliano Susana M, Director Ariel, Miragaya Marcelo H, Chaves María G, Sarchi María I, Stivale Diana, Quintans Carlos, Agüero Alicia, Rutter Bruno, Pasqualini Sergio
Аннотация
The interest for South American camelids has increased in the last years. The aim of the present research was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen samples were obtained by electroejaculation and incubated at 38 degrees C in a 25% (v/v) collagenase solution. The cleavage and embryo development rates were compared between the different experimental groups. Only the number of cleaved oocytes was less when ICSI with no activation was used (p<0.05).