Species specificity of guinea pig and human lymphotoxin colony inhibitory activity.

The species specificity of lymphotoxin colony inhibitory activity was measured by in vitro colony growth of neoplastic and nonneoplastic cells in the presence of lymphotoxin from the same or from a xenogeneic species. Growth of guinea pig, rat, and mouse neoplastically transformed cells was inhibited by guinea pig lymphotoxin. Colony formation of benzo(a)pyrene transformed 104C1 guinea pig cells was inhibited 45% with lymphotoxin at 42 units/ml medium; nontransformed 104 and 118 guinea pigs cell were not inhibited. Alpha L929, a spontaneously transformed mouse cell, was completely inhibited. Dimethylbenz(a)anthracene (DMBA) 3T3-D and R4-2 mouse transformed cells were inhibited 40 and 100%, respectively, but parental nontransformed 3T3 cells were not inhibited. DMBA transformed R-2 and R-6 rat cells were inhibited 20 and 40%, respectively, but secondary nontransformed fetal rat cells were unaffected. In contrast, normal human xeroderma pigmentosum, Lesch-Nyhan and 1220 fibroblast cells were inhibited 50-60%. No inhibition of HeLa or RPMI 2650 human tumor cells occurred. HeLa and RPMI 2650 cells, however, were inhibited 30 and 60%, respectively, by human lymphotoxin, but normal human and tumorigenic guinea pig cells were unaffected. Thus, guinea pig and human lymphotoxin exhibit species specific and differential cytotoxicity mechanisms for normal and tumor cells.