Future medicinal chemistry · 2018
Suramin potently inhibits cGAMP synthase, cGAS, in THP1 cells to modulate IFN-β levels.
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Wang Modi, Sooreshjani Moloud A, Mikek Clinton, Opoku-Temeng Clement, Sintim Herman O
Аннотация
AIM: Persistent activation of STING pathway is the basis for several autoimmune diseases. STING is activated by cGAMP, which is produced by cGAS in the presence of DNA. Results/methodology: HPLC-based medium throughput screening for inhibitors of cGAS identified suramin as a potent inhibitor. Unlike other reported cGAS inhibitors, which bind to the ATP/GTP binding site, suramin displaced the bound DNA from cGAS. Addition of suramin to THP1 cells reduced the levels of IFN-β mRNA and protein. Suramin did not inhibit lipopolysaccharide- or Pam3CSK4-induced IL-6 mRNA expression.
CONCLUSION: Suramin inhibits STING pathway via the inhibition of cGAS enzymatic activity. Suramin or analogs thereof that displace DNA from cGAS could be used as anti-inflammatory drugs.
CONCLUSION: Suramin inhibits STING pathway via the inhibition of cGAS enzymatic activity. Suramin or analogs thereof that displace DNA from cGAS could be used as anti-inflammatory drugs.
Предметные рубрики MeSH
Anti-Inflammatory AgentsAutoimmune DiseasesEnzyme InhibitorsGene Expression RegulationHumansInterferon-betaMembrane ProteinsMolecular StructureNucleotidyltransferasesRNA, MessengerSignal TransductionStructure-Activity RelationshipSuraminTHP-1 CellsCyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase