Future microbiology · 2016
Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization.
СтатьяНазвание и аннотация: Перевод в очереди
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Xu Qingqiang, Cao Mingmei, Song Hongyuan, Chen Shenglin, Qian Xijing, Zhao Ping, Ren Hao, Tang Hailin, Wang Yan, Wei Youheng, Zhu Yongzhe, Qi Zhongtian
Аннотация
AIM: To investigate the detailed mechanism of Japanese encephalitis virus (JEV) cell entry.
MATERIALS & METHODS: Utilize a siRNA library targeting cellular membrane trafficking genes to identify key molecules that mediate JEV entry into human neuronal cells.
RESULTS: JEV enters human neuronal cells by caveolin-1-mediated endocytosis, which depends on a two-step regulation of actin cytoskeleton remodeling triggered by RhoA and Rac1: RhoA activation promoted the phosphorylation of caveolin-1, and then Rac1 activation facilitated caveolin-associated viral internalization. Specifically, virus attachment activates the EGFR-PI3K signaling pathway, thereby leading to RhoA activation.
CONCLUSION: This work provides a detailed picture of the entry route and intricate cellular events following the entry of JEV into human neuronal cells, and promotes a better understanding of JEV entry.
MATERIALS & METHODS: Utilize a siRNA library targeting cellular membrane trafficking genes to identify key molecules that mediate JEV entry into human neuronal cells.
RESULTS: JEV enters human neuronal cells by caveolin-1-mediated endocytosis, which depends on a two-step regulation of actin cytoskeleton remodeling triggered by RhoA and Rac1: RhoA activation promoted the phosphorylation of caveolin-1, and then Rac1 activation facilitated caveolin-associated viral internalization. Specifically, virus attachment activates the EGFR-PI3K signaling pathway, thereby leading to RhoA activation.
CONCLUSION: This work provides a detailed picture of the entry route and intricate cellular events following the entry of JEV into human neuronal cells, and promotes a better understanding of JEV entry.
Предметные рубрики MeSH
Actin CytoskeletonActin Depolymerizing FactorsAnimalsCaveolin 1Cell LineCell MembraneCholesterolCricetinaeDynamin IIEncephalitis Virus, JapaneseEncephalitis, JapaneseEndocytosisErbB ReceptorsHEK293 CellsHost-Pathogen InteractionsHumansLife Cycle StagesMembrane Transport ProteinsNeuronsPhosphatidylinositol 3-KinasesPhosphorylationProtein-Tyrosine KinasesRNA, Small InterferingSignal TransductionTransfectionVirus AttachmentVirus Internalizationrac1 GTP-Binding ProteinrhoA GTP-Binding Protein