[Effects of Pa-pex 11 gene on penicillin production in Penicillium aurantiogriseum].

OBJECTIVE

To study the effect of Pa-pex 11 gene on penicillin production in Pencillium aurantiogriseum.

METHOD: Based on the conserved domain of pex 11 from Penicillium chrysogenum, we designed primers pex01 and pex02 to amplify Pa-pex 11 gene from P. aurantiogriseum. The complete Pa-pex 11 gene was cloned via thermal asymmetric interlaced PCR (Tail-PCR). In order to obtain tranformants with additional Pa-pex 11 gene copy, ATMT (Agrobacterium tumefaciens mediated transformation) was used to transform P. aurantiogriseum. The copy number of Pa-pex 11 was measured by quantitative PCR. Penicillin production of the transformant was analyzed by bioassays and the microbodies were observed by transmission electron microscope (TEM).

RESULTS

ATMT was successfully applied in P. aurantiogriseum. Increasing the copy number of Pa-pex 11 gene resulted in a 1.7-fold increase of pencillin production, and the electron microscope graph showed that the number of microbodies increased obviously.

CONCLUSION

Our results suggest that increasing the copy number of Pa-pex 11 can increase the number of microbodies, and stimulate the penicillin production.