Bioanalysis · 2024
Simultaneous estimation of rutin and donepezil through RP-HPLC: implication in pharmaceutical and biological samples.
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Rana Rafquat, Mishra Keerti, Tripathi Shourya, Gupta Animesh Kumar, Tiwari Amrendra Kumar, Yadav Pavan Kumar, Kumar Abhiram, Chakradhar Jvus, Singh Sanjay, Verma Sonia, Yadav Pooja, Chourasia Manish K
Аннотация
Aim: A HPLC method was developed and validated for the novel combination of rutin (RN) and donepezil (DNP). Materials & methods: RN and DNP were simultaneously eluted through a C18 column (Ø 150 × 4.6 mm) with a 60:40 v/v ratio of 0.1% formic acid aqueous solution to methanol at 0.5 ml/min. Results: The purposed method was found linear, selective, reproducible, accurate and precise with percent RSD less than 2. The limit of quantification for RN and DNP was found 3.66 and 3.25 μg/ml, respectively. Conclusion: Validated as per the ICH guidelines, the developed method efficiently quantified RN and DNP co-loaded in DQAsomes (121 nm) estimating matrix effect, release profile, entrapment efficiency, loading efficiency and in vivo plasma kinetics.