Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment · 2012
A sensitive chromatographic strip test for the rapid detection of enrofloxacin in chicken muscle.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАВалидационное исследованиеНазвание и аннотация: Перевод в очереди
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Chen Xuelan, Xu Hengyi, Lai Weihua, Chen Yuan, Yang Xiaohui, Xiong Yonghua
Аннотация
A sensitive colloidal gold immunochromatography assay using a specific monoclonal antibody was developed for the rapid detection of enrofloxacin (ENR) residues in chicken muscles. Anti-ENR antibodies with high sensitivity and specificity are generated by immunising BALB/c mice with well-characterised ENR-bovine serum albumin conjugate. An orthogonal L₉(3)³ test was designed, and various parameters that influenced the assay performance were investigated and optimised. Under the optimised conditions, the cut-off limits of semi-quantitative test strips for ENR were found to be 3 ng/mL in phosphate-buffered saline and 8 µg/kg in chicken muscle. The ENR test strips showed a 6% cross-reactivity with ciprofloxacin, 3% with norfloxacin, less than 1% with ofloxacin and sarafloxacin and 0.1% with the other eight fluoroquinolones including enoxacin, difloxacin, danofloxacin, pefloxacin, lomefloxacin, sparfloxacin, oxolinic acid and flumequine. Consistent results are produced from the parallel analysis of ENR-contaminated chicken muscle extracts using test strips and ELISA.