Generation of cancer-specific CD8(+) CD69(+) cells inhibits colon cancer growth.

The therapy of colon cancer (Cca) is poor currently. The abnormality of immune function is recognized in cancer-bearing hosts. This study aims to inhibit Cca growth with the in vivo induction of Cca-specific CD8(+) CD69(+) T cell polarization. In this study, a mouse tumor model was created with a mouse colon cancer (Cca) cell line. The Cca-bearing mice were immunized with Cca extracts and alum (using as an adjuvant). The CD8(+) CD69(+) cell polarization status was analyzed by flow cytometry and enzyme-linked immunosorbent assay. The results showed that a Cca antigen-specific CD8(+) CD69(+) cells were induced in the Cca-bearing mice. The Cca growth was efficiently inhibited by the Cca-specific CD8(+) CD69(+) cell response. The Cca-specific CD8(+) CD69(+) cells were detected in the Cca mass and spleen, which had the ability to induce Cca cell apoptosis. In conclusion, the Cca-specific CD8(+) CD69(+) cell polarization can be induced in Cca-bearing mice, which can efficiently inhibit the Cca growth.